1QHH

STRUCTURE OF DNA HELICASE WITH ADPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS
Synchrotron site	SRS
Temperature [K]	287
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 65
Unit cell lengths	138.936, 138.936, 111.642
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.228 *
Rwork	0.228
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	3.047
Data reduction software	DENZO
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.500
Rmerge	0.103
Number of reflections	42105
Completeness [%]	92.5
Redundancy	2.36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	20 *	Bird, L.E., (1998) Nucl. Acids Res., 26, 2686. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris	50 (mM)
3	1	drop		300 (mM)
4	1	reservoir	MES	100 (mM)
5	1	reservoir	sodium acetate	1.0 (M)