1QH5

HUMAN GLYOXALASE II WITH S-(N-HYDROXY-N-BROMOPHENYLCARBAMOYL)GLUTATHIONE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	1998-11-01
Detector	ADSC
Spacegroup name	P 21 21 21
Unit cell lengths	39.550, 73.800, 164.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.450
R-factor	0.204
Rwork	0.204
R-free	0.23200
Structure solution method	OTHER
RMSD bond length	0.012
RMSD bond angle	0.027
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.000	1.480
High resolution limit [Å]	1.450	1.450
Rmerge	0.115	0.340
Total number of observations	437671 *
Number of reflections	84815 *
<I/σ(I)>	13	4
Completeness [%]	99.0	100
Redundancy	5.2	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.1 *		EQUILABRATION AGAINST 4-20% W/V PEG 2000 MONOMETHYL ETHER, pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	MOPS	10 (mM)
3	1	drop	PEG400	25 (%)
4	1	reservoir	mPEG2000	4-20 (%)
5	1	reservoir	sodium imidazole	0.1 (M)
6	1	reservoir	dithiothreitol	2 (mM)