1QGR
STRUCTURE OF IMPORTIN BETA BOUND TO THE IBB DOMAIN OF IMPORTIN ALPHA (II CRYSTAL FORM, GROWN AT LOW PH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.896, 101.167, 83.831 |
| Unit cell angles | 90.00, 87.86, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.300 |
| R-factor | 0.235 * |
| Rwork | 0.235 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB IDCODE 1QGK |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.089 | 0.380 |
| Number of reflections | 46295 | |
| <I/σ(I)> | 13.7 | 2 |
| Completeness [%] | 98.5 | 98.5 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 3.9 | pH 3.9 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 16-20 (%) | |
| 2 | 1 | 1 | potassium phosphate | 50 (mM) | |
| 3 | 1 | 1 | beta-mercaptoethanol | 10 (mM) | |
| 4 | 1 | 1 | sodium iodide | 5 (mM) | |
| 5 | 1 | 1 | protain | 50 (mg/ml) |
