1QGI

CHITOSANASE FROM BACILLUS CIRCULANS

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	293
Wavelength(s)	1.0000,1.0721,1.0722,1.0728
Spacegroup name	P 21 21 2
Unit cell lengths	43.300, 128.000, 57.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 1.600
Rwork	0.192
R-free	0.23500
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	22.000 *
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Rmerge	0.085	0.656 *
Total number of observations	462029 *
Number of reflections	40769
Completeness [%]	95.1	83.7 *
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.2	293 *	pH 7.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	65 (%(w/v))
2	1	reservoir	ammonium sulfate	75 (%(w/v))