1QG2

CANINE GDP-RAN R76E MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-06-21
Detector	MAR scanner 345 mm plate
Spacegroup name	P 41 21 2
Unit cell lengths	102.320, 102.320, 42.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.198 *
Rwork	0.174
R-free	0.24600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	EBI-1279
RMSD bond length	0.011 *
RMSD bond angle	2.000 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.112	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.066	0.343
Number of reflections	9871
<I/σ(I)>	9.4	2.1
Completeness [%]	95.5	91.9
Redundancy	2.5 *	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	18 *	SEE PUBLICATION, pH 7.20

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG1000	20-36 (%(v/v))
3	1	reservoir		20 (mM)
4	1	reservoir	Tris-HCl	50 (mM)