1QFG
E. COLI FERRIC HYDROXAMATE RECEPTOR (FHUA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 171.550, 171.550, 87.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.300 - 2.500 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27100 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 28.300 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.300 | 2.500 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.078 | 0.334 |
| Number of reflections | 53749 | |
| <I/σ(I)> | 17 | |
| Completeness [%] | 99.3 | 92.2 |
| Redundancy | 6.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 290 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.5 (mg/ml) | |
| 2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 3 | 1 | reservoir | mPEG2000 | 11 (%) | |
| 4 | 1 | reservoir | glycerol | 20 (%) | |
| 5 | 1 | reservoir | PEG200 | 3 (%) | |
| 6 | 1 | reservoir | dimethyldecylamine-N-oxide | 0.8 (%) | |
| 7 | 1 | reservoir | cis-inositol | 1 (%) |
