1QFF
E. COLI FERRIC HYDROXAMATE UPTAKE RECEPTOR (FHUA) IN COMPLEX WITH BOUND FERRICHROME-IRON
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 61 |
| Unit cell lengths | 171.400, 171.400, 85.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.27600 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.940 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 39663 | |
| Completeness [%] | 98.9 | 98 |
| Redundancy | 5.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.4 | 290 * | pH 6.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.5 (mg/ml) | |
| 2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 3 | 1 | reservoir | mPEG2000 | 11 (%) | |
| 4 | 1 | reservoir | glycerol | 20 (%) | |
| 5 | 1 | reservoir | PEG200 | 3 (%) | |
| 6 | 1 | reservoir | dimethyldecylamine-N-oxide | 0.8 (%) | |
| 7 | 1 | reservoir | cis-inositol | 1 (%) |
