1QCI
LOW TEMPERATURE STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN COMPLEXED WITH ADENINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-05 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.480, 49.030, 63.870 |
| Unit cell angles | 68.79, 81.05, 63.88 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.23 * |
| Rwork | 0.230 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.098 |
| Number of reflections | 30085 | |
| Completeness [%] | 90.6 | 83.3 |
| Redundancy | 2.3 | 1.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 16-18% PEG 4000, 0.1M CACL2, 5MM ADENYL(3'-5')GUANOSINE, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-20 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 16-18 (%) | |
| 3 | 1 | reservoir | 0.1 (M) | ||
| 4 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 5 | 1 | reservoir | ApG | 5 (mM) |
