1QCA

QUADRUPLE MUTANT Q92C, N146F, Y168F, I172V TYPE III CAT COMPLEXED WITH FUSIDIC ACID. CRYSTALS GROWN AT PH 6.3. X-RAY DATA COLLECTED AT ROOM TEMPERATURE

Experimental procedure

Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1992-08-27
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	107.810, 107.810, 124.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.174
RMSD bond length	0.018
RMSD bond angle	0.049
Data reduction software	MOSFLM
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	15.600
High resolution limit [Å]	2.200
Rmerge	0.095
Total number of observations	68511 *
Number of reflections	14238
Completeness [%]	99.0
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	6.3	4 *	pH 6.3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	5.7 (mg/ml)
2	1	1	MES	10 (mM)	or supplemented with 0.5 mM sodium fusidate
3	1	2	MES	10 (mM)
4	1	2	MPD	2-4 (%(v/v))
5	1	2	hexamine cobalt(III) chloride	1 (mM)
6	1	2	chloramphenicol	1 (mM)	can be replaced by 0.5mM sodium fusidate
7	1	2	dithiothreitol	0.1 (mM)