1QB8
CRYSTAL STRUCTURES OF ADENINE PHOSPHORIBOSYLTRANSFERASE FROM LEISHMANIA DONOVANI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 64.040, 64.040, 235.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.000 - 2.000 |
| Rwork | 0.196 |
| R-free | 0.26300 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 * | |
| High resolution limit [Å] | 2.000 * | |
| Rmerge | 0.053 * | 0.175 * |
| Total number of observations | 328256 * | |
| Number of reflections | 17882 * | |
| Completeness [%] | 87.9 * | 60.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 4 * | PEG 5000 MME, AMMONIUM ACETATE, SODIUM CITRATE, MAGNESIUM CHLORIDE, ADENOSINE- 5-MONOPHOSPHATE (AMP), pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | MES | 10 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | reservoir | AMP | 10 (mM) | |
| 6 | 1 | reservoir | mPEG5000 | 7-11 (%) | |
| 7 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 8 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 9 | 1 | reservoir | 10 (mM) |
