1QB3

CRYSTAL STRUCTURE OF THE CELL CYCLE REGULATORY PROTEIN CKS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-10-20
Detector	MARRESEARCH
Spacegroup name	P 62 2 2
Unit cell lengths	105.810, 105.810, 165.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 3.000
R-factor	0.216 *
Rwork	0.216
R-free	0.29100
RMSD bond length	0.015
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.094	0.405
Total number of observations	243609 *
Number of reflections	10366 *
<I/σ(I)>	7.4
Completeness [%]	91.0	93.7
Redundancy	12.6	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	20 *	20% PEG 4K, 5% NASCN AND 0.1 M IMIDAZOLE/MALATE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	PEG4000	20 (%)
3	1	drop		5 (%sat)
4	1	drop	imidazole/malate	0.1 (M)