1QAT
1-PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE PHOSPHODIESTERASE DELTA COMPLEX WITH SAMARIUM (III) CHLORIDE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.600, 75.100, 86.400 |
| Unit cell angles | 66.40, 85.60, 89.80 |
Refinement procedure
| Resolution | 6.000 - 3.000 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.26100 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | APO TRICLINIC PHOSPHOLIPASE C |
| RMSD bond length | 0.009 |
| RMSD bond angle | 24.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.121 | 0.248 |
| Number of reflections | 24235 | |
| <I/σ(I)> | 7.2 | 2.3 |
| Completeness [%] | 84.9 | 73.8 |
| Redundancy | 2.5 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | used to seeding, Grobler, J.A., (1996) Protein Sci., 5, 680. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | NaMes | 0.1 (M) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | reservoir | glycerol | 20 (%) | |
| 4 | 1 | reservoir | PEG8000 | 6-8 (%) |
