1QAJ
CRYSTAL STRUCTURES OF THE N-TERMINAL FRAGMENT FROM MOLONEY MURINE LEUKEMIA VIRUS REVERSE TRANSCRIPTASE COMPLEXED WITH NUCLEIC ACID: FUNCTIONAL IMPLICATIONS FOR TEMPLATE-PRIMER BINDING TO THE FINGERS DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.868, 63.587, 73.398 |
| Unit cell angles | 90.00, 102.91, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.25900 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.140 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.048 | 0.174 |
| Total number of observations | 91393 * | |
| Number of reflections | 26120 | |
| <I/σ(I)> | 21.3 | |
| Completeness [%] | 98.7 | 97.1 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Sun, D., (1998) Protein Sci., 7, 1575. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NH4CL | ||
| 2 | 1 | 1 | HEPES | ||
| 3 | 1 | 1 | PEG 4000 | ||
| 4 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 100 (mM) | |
| 2 | 1 | reservoir | PEG4000 | 13 (%) | |
| 3 | 1 | reservoir | 75 (mM) |
