1QAB

The structure of human retinol binding protein with its carrier protein transthyretin reveals interaction with the carboxy terminus of RBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1996-05-15
Detector	ADSC
Spacegroup name	P 41 21 2
Unit cell lengths	140.230, 140.230, 124.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.200 - 3.200
R-factor	0.278 *
Rwork	0.278
R-free	0.40300
RMSD bond length	0.011
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall
High resolution limit [Å]	3.200
Rmerge	0.117
Total number of observations	331570 *
Number of reflections	26640
Completeness [%]	99.9
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Hepes
2	1	1	Li2SO4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	17 (mg/ml)
2	1	reservoir		1.8 (M)
3	1	reservoir	HEPES	40 (mM)