1Q9Y
CRYSTAL STRUCTURE OF ENTEROBACTERIA PHAGE RB69 GP43 DNA POLYMERASE COMPLEXED WITH 8-OXOGUANOSINE CONTAINING DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-18 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.822, 118.565, 127.224 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.060 - 2.800 |
| R-factor | 0.20726 |
| Rwork | 0.204 |
| R-free | 0.27067 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ig9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.865 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 30441 | |
| <I/σ(I)> | 11.0777 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.5 | 1.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 350MME, Calcium chloride, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.50 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 350MME | ||
| 2 | 1 | 1 | Calcium chloride | ||
| 3 | 1 | 1 | Tris-HCl | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 350MME | ||
| 6 | 1 | 2 | Calcium chloride | ||
| 7 | 1 | 2 | H2O |
