1Q9X
Crystal structure of Enterobacteria phage RB69 gp43 DNA polymerase complexed with tetrahydrofuran containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-10 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 131.948, 122.237, 165.358 |
| Unit cell angles | 90.00, 96.85, 90.00 |
Refinement procedure
| Resolution | 47.000 - 2.690 |
| R-factor | 0.21879 |
| Rwork | 0.215 |
| R-free | 0.28790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1clq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.535 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.800 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Number of reflections | 137338 | |
| <I/σ(I)> | 19.1 | 1.8 |
| Completeness [%] | 94.7 | 73.3 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 298 | PEG 350MME, Calcium chloride, Tris-HCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.50 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 350 | ||
| 2 | 1 | 1 | Calcium chloride | ||
| 3 | 1 | 1 | Tris-HCl | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 350 | ||
| 6 | 1 | 2 | Calcium chloride | ||
| 7 | 1 | 2 | Tris-HCl | ||
| 8 | 1 | 2 | H2O |
