1Q9E

RNase T1 variant with adenine specificity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Spacegroup name	P 61
Unit cell lengths	56.500, 56.500, 158.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 1.700
R-factor	0.199
Rwork	0.199
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ch0
RMSD bond length	0.015
RMSD bond angle	24.960 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	20.000
High resolution limit [Å]	1.700	1.700
Rmerge	0.037 *
Total number of observations	70573 *
Number of reflections	31400 *
Completeness [%]	90.6	95.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	279	PEG4000, magnesium chloride, Tris, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 279K, pH 9.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNase T1 RV	2 (mM)
2	1	drop	Tris-HCl	10 (mM)	pH8.5
3	1	reservoir	Tris-HCl	100 (mM)	pH9.0
4	1	reservoir		200 (mM)
5	1	reservoir	PEG4000	20-22 (%)