1Q9E
RNase T1 variant with adenine specificity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Spacegroup name | P 61 |
| Unit cell lengths | 56.500, 56.500, 158.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 1.700 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ch0 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 24.960 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.037 * | |
| Total number of observations | 70573 * | |
| Number of reflections | 31400 * | |
| Completeness [%] | 90.6 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | 279 | PEG4000, magnesium chloride, Tris, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 279K, pH 9.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNase T1 RV | 2 (mM) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.5 |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH9.0 |
| 4 | 1 | reservoir | 200 (mM) | ||
| 5 | 1 | reservoir | PEG4000 | 20-22 (%) |
