1Q9C
Crystal Structure of the Histone domain of Son of Sevenless
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.397, 109.199, 212.399 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 3.210 |
| Rwork | 0.255 |
| R-free | 0.29200 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 18.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 * | 3.310 |
| High resolution limit [Å] | 3.200 * | 3.210 |
| Rmerge | 0.066 * | 0.365 * |
| Number of reflections | 35294 | |
| <I/σ(I)> | 20.1 | 4 |
| Completeness [%] | 99.0 * | 96.9 * |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG3350, L-proline, magnesium acetate, ethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-20 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 4-12 (%) | |
| 3 | 1 | reservoir | L-proline | 0.2 (M) | |
| 4 | 1 | reservoir | magnesium acetate | 0.1 (M) | |
| 5 | 1 | reservoir | ethanol | 5 (%) |
