1Q97

The structure of the Saccharomyces cerevisiae SR protein kinase, Sky1p, with bound ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	94
Detector technology	CCD
Collection date	2002-06-15
Detector	SBC-2
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	71.823, 88.692, 133.495
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.890 - 2.300
Rwork	0.215
R-free	0.25630 *
RMSD bond length	0.007 *
RMSD bond angle	1.270 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.890	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.081 *	0.544 *
Total number of observations	178325 *
Number of reflections	33170
Completeness [%]	86.0	83.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	Nolen, B., (2001) Nat.Struct.Biol., 8, 176. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	ATP	0.0066 (mM)
3	1	drop		0.0017 (mM)
4	1	reservoir	ammonium sulfate	1.5 (M)
5	1	reservoir	ethylene glycol	15 (%(v/v))
6	1	reservoir	sodium acetate	10 (mM)