1Q87

Crystal structure of the C-domain of the T.vaginalis Inr binding protein, IBP39 (tetragonal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-12
Detector	ADSC QUANTUM 4
Wavelength(s)	1.08
Spacegroup name	P 41 21 2
Unit cell lengths	76.420, 76.420, 157.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.000 - 2.320
R-factor	0.215
Rwork	0.215
R-free	0.27500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q88 C-domain from the C2 monoclinic form
RMSD bond length	0.013
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.000	2.380
High resolution limit [Å]	2.320	2.320
Rmerge	0.042	0.386
Total number of observations	111463 *
Number of reflections	19164
<I/σ(I)>		2
Completeness [%]	91.6	91.6
Redundancy	5.8	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	298	PEG 5000, ammonium sulphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-50 (mg/ml)
2	1	reservoir	ammonium sulfate	200 (mM)
3	1	reservoir	MES	50 (mM)	pH6.5
4	1	reservoir	PEG5000	25 (%)