1Q7E

Crystal Structure of YfdW protein from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-23
Detector	MARRESEARCH
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	92.366, 69.605, 73.219
Unit cell angles	90.00, 108.63, 90.00

Refinement procedure

Resolution	20.000 - 1.600
Rwork	0.156
R-free	0.18600
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	56025
<I/σ(I)>	11.8	4
Completeness [%]	97.5	95.8
Redundancy	3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	70% MPD, 0.1 M Bis-Tris pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	70% MPD, 0.1 M Bis-Tris pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	70% MPD, 0.1 M Bis-Tris pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K