1Q62
PKA double mutant model of PKB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 290 |
| Detector technology | AREA DETECTOR |
| Collection date | 2001-07-17 |
| Detector | BRUKER |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.302, 75.267, 80.036 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.19242 |
| Rwork | 0.185 |
| R-free | 0.25198 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.764 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.220 |
| Number of reflections | 19181 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | MES-Bistris, LiCl, DTT, Mega8, PKI, methanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
