1Q4R

Gene Product of At3g17210 from Arabidopsis Thaliana

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	90
Detector technology	CCD
Collection date	2003-06-26
Detector	MARRESEARCH
Wavelength(s)	0.979
Spacegroup name	P 62
Unit cell lengths	55.468, 55.468, 57.673
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.18469
Rwork	0.182
R-free	0.23200 *
Structure solution method	MAD
RMSD bond length	0.020
RMSD bond angle	1.861
Data scaling software	SCALEPACK
Phasing software	SOLVE (RESOLVE)
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.042	0.274 *
Total number of observations	75176 *
Number of reflections	14162 *
<I/σ(I)>	35.6	2.06
Completeness [%]	93.5 *	52.6 *
Redundancy	5.2 *	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	297	Sodium MES, Sodium citrate, MEthyl Ether PEG 5000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	mPEG5000	30 (%(w/v))
2	1	reservoir	sodium cirtate	120 (mM)
3	1	reservoir	sodium HEPES	100 (mM)	pH6.0
4	1	drop		100 (mM)
5	1	drop	sodium HEPES	10 (mM)	pH7.0
6	1	drop	protein	14 (mg/ml)