1Q46

crystal structure of the eIF2 alpha subunit from saccharomyces cerevisia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2003-03-20
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	94.009, 94.009, 81.198
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.860
R-factor	0.222
Rwork	0.218
R-free	0.24300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	human eIF2 alpha subunit
RMSD bond length	0.006 *
RMSD bond angle	1.210 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.960
High resolution limit [Å]	2.860	2.860
Rmerge	0.077 *	0.583 *
Total number of observations	77383 *
Number of reflections	9935 *
<I/σ(I)>		1.3
Completeness [%]	99.5 *	99.9
Redundancy	7.78 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	293	0.1 M sodium acetate (pH=4.6), 1.8-2.5 M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium formate	1.8-2.5 (M)
2	1	1	acetate	0.1 (M)