1Q40
Crystal structure of the C. albicans Mtr2-Mex67 M domain complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-01-24 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9881,0.9809,0.815 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.414, 54.077, 109.751 |
Unit cell angles | 90.00, 96.12, 90.00 |
Refinement procedure
Resolution | 12.000 - 1.950 |
R-factor | 0.17775 |
Rwork | 0.177 |
R-free | 0.22600 * |
Structure solution method | MAD |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.190 * |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.053 * | 0.311 * |
Total number of observations | 530670 * | |
Number of reflections | 56333 * | |
<I/σ(I)> | 9.5 | 2.3 |
Completeness [%] | 91.6 | 67 |
Redundancy | 4.3 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 | 20 * | PEG 4000, imidazol-malate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 6.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 18 (%) | |
2 | 1 | reservoir | imidazole-malate | 0.2 (M) | pH6.0 |
3 | 1 | drop | protein | 9.5 (mg/ml) |