1Q3I
Crystal Structure of Na,K-ATPase N-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.094 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 147.310, 147.310, 147.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| Rwork | 0.251 |
| R-free | 0.29200 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.390 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.075 | 0.333 |
| Number of reflections | 8238 | |
| <I/σ(I)> | 9.3 | 2.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | Ammonium sulphate (1.7-2M), TrisHCl (50mM), NiCl2(1mM), pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
