1Q33
Crystal structure of human ADP-ribose pyrophosphatase NUDT9
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 170 |
Detector technology | CCD |
Collection date | 2002-03-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.997 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.837, 87.497, 119.814 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.540 - 1.810 |
R-factor | 0.2037 |
Rwork | 0.201 |
R-free | 0.23130 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.450 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.760 | 1.830 |
High resolution limit [Å] | 1.810 | 1.800 |
Number of reflections | 36910 | |
<I/σ(I)> | 45.8 | 5.54 |
Completeness [%] | 94.1 | 66.2 |
Redundancy | 9.3 | 2.98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | Ammonium sulfate, TrisHCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |