1Q2Y
Crystal structure of the protein YJCF from Bacillus subtilis: a member of the GCN5-related N-acetyltransferase superfamily fold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-24 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0088 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.266, 59.283, 71.556 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.000 |
R-factor | 0.239 |
Rwork | 0.234 |
R-free | 0.26500 |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 1.360 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.090 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.048 | 0.153 |
Number of reflections | 9584 | |
<I/σ(I)> | 32.6 | 7.8 |
Completeness [%] | 93.0 | 92.1 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 550, Ammonium tartrate, BisTris , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |