1Q2D
Crystal Structure of Tetrahymena GCN5 With Bound Coenzyme A and a 19-residue p53 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Detector technology | CCD |
Collection date | 2000-06-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9213 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.740, 64.740, 96.413 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.880 - 2.250 |
Rwork | 0.247 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qsn |
RMSD bond length | 0.009 |
RMSD bond angle | 1.515 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.800 |
High resolution limit [Å] | 1.900 |
Number of reflections | 23773 |
Completeness [%] | 87.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Ammonium sulfate, Hepes, Sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |