1Q24
PKA double mutant model of PKB in complex with MgATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 280 |
| Detector technology | AREA DETECTOR |
| Collection date | 2002-02-04 |
| Detector | BRUKER |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.286, 75.387, 80.377 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.050 - 2.600 |
| R-factor | 0.202 |
| Rwork | 0.196 |
| R-free | 0.24100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.050 | |
| High resolution limit [Å] | 2.600 | |
| Rmerge | 0.100 * | 0.390 * |
| Number of reflections | 11741 | |
| Completeness [%] | 86.0 * | 72 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.4 | 5 * | Mes-Bistris, LiCl, DTT, Mega8, PKI, ATP, MgCl, , pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 277.16K, pH 6.40 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 10 | 1 | reservoir | methanol | 12-18 (%(v/v)) | |
| 2 | 1 | drop | MES-Bis Tris | 25 (mM) | |
| 3 | 1 | drop | 75 (mM) | ||
| 4 | 1 | drop | 0.1 (mM) | ||
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | drop | octanoyl-N-methylglucamide | 1.5 (mM) | |
| 7 | 1 | drop | PKI-(5-24) | 1 (mM) | pH6.3-6.6 |
| 8 | 1 | drop | ATP | 1.5 (mM) | |
| 9 | 1 | drop | 3 (mM) |
