1Q1H
An extended winged helix domain in general transcription factor E/IIE alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 66.100, 66.100, 92.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.500 - 2.900 |
| R-factor | 0.3151 |
| Rwork | 0.315 |
| R-free | 0.32100 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.330 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.900 | 2.900 * |
| Rmerge | 0.077 * | 0.285 * |
| Number of reflections | 3025 | 292 * |
| Completeness [%] | 98.7 | 100 * |
| Redundancy | 9.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | TRIS-Cl, sodium acetate, PEG 1000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 50 (mM) | pH7.5 |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | 10000 (nM) | ||
| 5 | 1 | drop | dithiothreitol | 5 (mM) | |
| 6 | 1 | drop | protein | 15 (mg/ml) | |
| 7 | 1 | reservoir | Tris-Cl | 100 (mM) | pH8.5 |
| 8 | 1 | reservoir | 200 (mM) | ||
| 9 | 1 | reservoir | PEG4000 | 30 (%(w/v)) |
