1Q1F
Crystal structure of murine neuroglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2, 1.7 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 86.973, 86.973, 110.815 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.21 |
| Rwork | 0.184 |
| R-free | 0.20750 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.074 | 0.536 |
| Number of reflections | 26076 | |
| <I/σ(I)> | 35 | 2.79 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 10.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 M Na acetate, 2M Na formate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
