1Q0T
Ternary Structure of T4DAM with AdoHcy and DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.700, 109.700, 73.600 |
| Unit cell angles | 90.00, 104.20, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| Rwork | 0.238 |
| R-free | 0.29300 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.100 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.096 | 0.152 |
| Total number of observations | 43112 * | |
| Number of reflections | 11038 | |
| <I/σ(I)> | 13.9 | 8.6 |
| Completeness [%] | 98.6 | 97.3 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 16 * | PEG 8000, ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | ammonium sulfate | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | PEG 8000 | ||
| 5 | 1 | 2 | ammonium sulfate | ||
| 6 | 1 | 2 | H2O |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 26 (mg/ml) | |
| 2 | 1 | reservoir | 2.4 (M) | ||
| 3 | 1 | reservoir | MES | 100 (mM) | pH6.0 |
