1Q0B
Crystal structure of the motor protein KSP in complex with ADP and monastrol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.300, 79.500, 159.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.22 |
| Rwork | 0.219 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ii6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.270 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.067 | 0.240 |
| Number of reflections | 70259 | |
| <I/σ(I)> | 26 | 4.5 |
| Completeness [%] | 99.0 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 277 | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 2 (mM) | ||
| 3 | 1 | drop | TCEP | 1 (mM) | |
| 4 | 1 | drop | ATP | 1 (mM) | |
| 5 | 1 | drop | 84 (mM) | ||
| 6 | 1 | drop | EGTA | 1 (mM) | |
| 7 | 1 | reservoir | PEG3350 | 20 (%(w/v)) | |
| 8 | 1 | reservoir | 150 (mM) | ||
| 9 | 1 | reservoir | HEPES | 100 (mM) | pH7. |
