1Q03
Crystal structure of FGF-1, S50G/V51G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | CCD |
| Collection date | 2003-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.900, 97.000, 108.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.440 - 2.050 |
| R-factor | 0.1971 |
| Rwork | 0.197 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.440 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.079 | 0.398 |
| Number of reflections | 20665 | |
| <I/σ(I)> | 4.7 | |
| Completeness [%] | 82.5 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | Formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
