1PZY
W314A-BETA1,4-GALACTOSYLTRANSFERASE-I COMPLEXED WITH ALPHA-LACTALBUMIN IN THE PRESENCE OF N-ACETYLGLUCOSAMINE, UDP AND MANGANESE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.292, 98.747, 102.788 |
| Unit cell angles | 90.00, 103.92, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.300 |
| R-factor | 0.2071 |
| Rwork | 0.207 |
| R-free | 0.25400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nmm |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.380 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 * | 0.480 * |
| Total number of observations | 161993 * | |
| Number of reflections | 47881 * | |
| <I/σ(I)> | 16.4 | 2.2 |
| Completeness [%] | 97.0 | 93.4 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | 11% PEG 4000, 0.1M Sodium chloride and 0.1M Sodium citrate, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.1 (M) | pH8.0 |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |
| 4 | 1 | reservoir | lithium sulfate | 0.1 (M) | |
| 5 | 1 | reservoir | polyethylene glycol | 30 (%(w/v)) |
