1PZN

Rad51 (RadA)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Detector technology	CCD
Collection date	2002-04-07
Detector	ADSC
Wavelength(s)	0.979029
Spacegroup name	C 2 2 21
Unit cell lengths	144.163, 193.124, 176.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 * - 2.850
Rwork	0.257
R-free	0.30700
Structure solution method	MAD
RMSD bond length	0.009 *
RMSD bond angle	1.390 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.094 *	0.370 *
Number of reflections	462283 *	57867 *
<I/σ(I)>	20.3	3.2198
Completeness [%]	97.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *
1	Vapor diffusion, sitting drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.2 (mg/ml)
2	1	drop	ATP	1 (mM)
3	1	drop		20 (mM)