1PZG
T.gondii LDH1 complexed with APAD and sulfate at 1.6 Angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-12-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.935, 124.978, 86.388 |
| Unit cell angles | 90.00, 105.94, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.19760 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pzf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.470 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASING (FROM ISOMORPHOUS STRUCTURE) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.036 | 0.346 |
| Total number of observations | 451492 * | |
| Number of reflections | 167180 | |
| <I/σ(I)> | 25.3 | 2.6 |
| Completeness [%] | 91.8 | 92.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.5 | 293 | 0.9 M ammonium sulfate, 100 mM acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | cosubstrate | 10 (mM) | |
| 3 | 1 | drop | oxalate | 5 (mM) | or oxamate |
| 4 | 1 | reservoir | ammonium sulfate | 0.85-0.90 (M) | |
| 5 | 1 | reservoir | acetate | 100 (mM) | pH4.5 |
