1PZC
APO-PSEUDOAZURIN (METAL FREE PROTEIN)
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Detector technology | IMAGE PLATE |
Collection date | 1994-07-13 |
Detector | MARRESEARCH |
Wavelength(s) | 0.87 |
Spacegroup name | P 65 |
Unit cell lengths | 49.500, 49.500, 99.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.850 |
R-factor | 0.164 |
R-free | 0.21500 |
RMSD bond length | 0.016 * |
RMSD bond angle | 0.040 * |
Data reduction software | DENZO |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 * |
High resolution limit [Å] | 1.850 * |
Rmerge | 0.077 |
Total number of observations | 73694 * |
Number of reflections | 11516 * |
Completeness [%] | 98.4 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5.7 * | Petratos, K., (1987) FEBS Lett., 218, 209. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1.5 (%) | |
2 | 1 | drop | 50 (mM) | ||
3 | 1 | drop | ammonium sulfate | 1.5 (M) | |
4 | 1 | reservoir | ammonium sulfate | 3.0 (M) |