1PZ4

The structural determination of an insect (mosquito) Sterol Carrier Protein-2 with a ligand bound C16 Fatty Acid at 1.35 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	AREA DETECTOR
Detector	Bruker X1000
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	29.300, 46.100, 70.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 1.350
R-factor	0.187
Rwork	0.187
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.234 *
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	EPMR
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	1.410
High resolution limit [Å]	1.350	1.350
Rmerge	0.038	0.252
Total number of observations	78412 *
Number of reflections	21157	2383 *
<I/σ(I)>		3.3
Completeness [%]	97.0	91.5 *
Redundancy	3.7	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2M sodium malonate, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		10 (mg/ml)
2	1	reservoir		2 (M)
3	1	reservoir		100 (mM)	pH7.5