1PYL
Crystal structure of Ribonuclease Sa2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-30 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.980, 34.130, 72.270 |
| Unit cell angles | 90.00, 109.50, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.507 |
| R-factor | 0.15009 |
| Rwork | 0.149 |
| R-free | 0.17230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rgg |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.540 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.063 | 0.272 |
| Number of reflections | 30643 | |
| <I/σ(I)> | 22.3 | 2.65 |
| Completeness [%] | 98.6 | 90.2 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | ammonium sulfate, phosphate buffer, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
