1PXY

Crystal structure of the actin-crosslinking core of Arabidopsis fimbrin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	200
Detector technology	CCD
Collection date	2001-03-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.984, 0.9786, 0.9789, 0.9560
Spacegroup name	P 1 21 1
Unit cell lengths	62.688, 104.981, 104.411
Unit cell angles	90.00, 103.93, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.2293
Rwork	0.229
R-free	0.26990
Structure solution method	MAD
Starting model (for MR)	1aoa
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge		0.315
Number of reflections	44865
<I/σ(I)>	20.71	3.35
Completeness [%]	87.5	86.7
Redundancy	5.97	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	298	6% PEG 8000, 100 mM Tris, 1mM DTT, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K