1PWO
Crystal Structure of Phospholipase A2 (MIPLA2) from Micropechis Ikaheka
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-01-01 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 129.186, 100.643, 88.307 |
Unit cell angles | 90.00, 133.17, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.600 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MiPLA4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.900 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 22385 | |
<I/σ(I)> | 4.52 | |
Completeness [%] | 97.8 | 99.2 |
Redundancy | 1.3 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 295 | 2.4M Ammonium Sulphate, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K |