1PWH

Rat Liver L-Serine Dehydratase- Complex with PYRIDOXYL-(O-METHYL-SERINE)-5-MONOPHOSPHATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-04-05
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	62.241, 109.262, 98.940
Unit cell angles	90.00, 91.66, 90.00

Refinement procedure

Resolution	25.000 * - 2.600
R-factor	0.23
Rwork	0.230
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Apo Enzyme 1PWE
RMSD bond length	0.009
RMSD bond angle	22.800 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000 *	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.078	0.124 *
Total number of observations	271545 *
Number of reflections	40796 *
<I/σ(I)>	1692.5	491.7
Completeness [%]	95.2	75.4
Redundancy	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	22 *	PEG 8000, potassium Acetate, TrisHCL, Dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 22K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	50 (mM)	pH8.0
2	1	reservoir	OMS	10 (mM)
3	1	reservoir	potassium acetate	200 (mM)
4	1	reservoir	dithiothreitol	1 (mM)
5	1	reservoir	PEG8000	14 (%(w/v))
6	1	reservoir	1,4-dioxane	2 (%)
7	1	drop	protein	20 (mg/ml)