1PWA

Crystal structure of Fibroblast Growth Factor 19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.975
Spacegroup name	H 3 2
Unit cell lengths	67.573, 67.573, 193.374
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.330 - 1.300
R-factor	0.1807
Rwork	0.180
R-free	0.19500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1JQZ 2fgf 1ijt 1qqk 1ihk
RMSD bond length	0.010
RMSD bond angle	1.440 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.330 *	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.062 *	0.586 *
Number of reflections	42303 *
<I/σ(I)>	36.5	4.32
Completeness [%]	99.9	100
Redundancy	12.08	11.30 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	PEG 3350, potassium phosphate, magnesium sulphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	10 (mM)
2	1	drop		200 (mM)
3	1	reservoir	PEG3350	12-14 (%(w/w))
4	1	reservoir		0.25 (M)
5	1	reservoir	potassium phosphate	0.1 (M)	pH7.0