1PW4

Crystal Structure of the Glycerol-3-Phosphate Transporter from E.Coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Detector technology	CCD
Collection date	2003-02-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9789, 1.214
Spacegroup name	P 32 2 1
Unit cell lengths	97.640, 97.640, 175.151
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 3.300
Rwork	0.296
R-free	0.32530
Structure solution method	SIRAS
RMSD bond length	0.010 *
RMSD bond angle	2.100 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	3.360
High resolution limit [Å]	3.300 *	3.300
Rmerge	0.060 *	0.386 *
Number of reflections	14870
<I/σ(I)>	11.3	2.8
Completeness [%]	99.5	93.2
Redundancy	7.7	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.7	293	25% PEG2K MME, 20% Glycerol, 5% MPD, 0.1M Tris pH8.7, 1mM DTT, 5mM NaAc, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	unknown *	8.7	293	25% PEG2K MME, 20% Glycerol, 5% MPD, 0.1M Tris pH8.7, 1mM DTT, 5mM NaAc, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	2 (mg/ml)
2	1	1	dithiothreitol	5 (mM)