1PVU

THE CRYSTAL STRUCTURE OF PVUII ENDONUCLEASE REVEALS EXTENSIVE STRUCTURAL HOMOLOGIES TO ECORV

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	84.650, 106.460, 47.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.400
R-factor	0.184
Rwork	0.184
RMSD bond length	0.023
RMSD bond angle	3.620
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.076 *
Number of reflections	16315 *
Completeness [%]	96.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	18 *	Athanasiadis, A., (1991) J.Mol.Biol., 222, 451. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein solution	2.5 (mg/ml)
2	1	drop	ammonium acetate	100 (mM)
3	1	drop	ammonium sulfate	10-20 (%)
4	1	reservoir	ammonium sulfate	44 (%)