1PVH
Crystal structure of leukemia inhibitory factor in complex with gp130
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.710, 86.700, 146.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 2.500 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.28800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | LIF - PDB ENTRY 1LKI GP130 - PDB ENTRY 1I1R |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.900 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.085 | 0.511 |
| Total number of observations | 463823 * | |
| Number of reflections | 35908 * | |
| <I/σ(I)> | 10.4 | 1.5 |
| Completeness [%] | 91.0 | 78.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | PEG 3350, Sodium iodide, Imidazole, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 8-10 (%) | |
| 3 | 1 | reservoir | sodium iodide | 0.2 (M) | |
| 4 | 1 | reservoir | imidazole | 0.1 (M) | pH7.5 |
