1PVH

Crystal structure of leukemia inhibitory factor in complex with gp130

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-15
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	79.710, 86.700, 146.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 * - 2.500
R-factor	0.248
Rwork	0.248
R-free	0.28800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	LIF - PDB ENTRY 1LKI GP130 - PDB ENTRY 1I1R
RMSD bond length	0.010
RMSD bond angle	22.900 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.630
High resolution limit [Å]	2.500	2.500
Rmerge	0.085	0.511
Total number of observations	463823 *
Number of reflections	35908 *
<I/σ(I)>	10.4	1.5
Completeness [%]	91.0	78.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	PEG 3350, Sodium iodide, Imidazole, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	reservoir	PEG3350	8-10 (%)
3	1	reservoir	sodium iodide	0.2 (M)
4	1	reservoir	imidazole	0.1 (M)	pH7.5