1PUE
PU.1 ETS DOMAIN-DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE D41A |
| Synchrotron site | LURE |
| Beamline | D41A |
| Temperature [K] | 130 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-01-21 |
| Detector | UCSD MARK III |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.100, 101.900, 55.600 |
| Unit cell angles | 90.00, 111.20, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.100 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.28700 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.630 |
| Data reduction software | MOSFLM |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Rmerge | 0.033 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 19 * | Pio, F., (1995) J.Biol.Chem., 270, 24258. * |
| 1 | unknown * | 6.5 | 19 * | Pio, F., (1995) J.Biol.Chem., 270, 24258. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | sodium cacodylate | 100 (mM) | |
| 2 | 1 | 1 | PEG600 | 3-10 (%) | |
| 3 | 1 | 1 | zinc acetate | 200 (mM) |
