1PTT

CRYSTAL STRUCTURE OF PROTEIN TYROSINE PHOSPHATASE 1B COMPLEXED WITH PHOSPHOTYROSINE-CONTAINING TETRA-PEPTIDE (AC-DEPYL-NH2)

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1994-10
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	88.500, 88.500, 124.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.900
R-factor	0.194
Rwork	0.194
RMSD bond length	0.021
RMSD bond angle	2.180
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.900
Rmerge	0.057
Total number of observations	86391 *
Number of reflections	10478
Completeness [%]	80.7
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *		4 *	Barford, D., (1994) J. Mol. Biol., 239, 726. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PTP1B	10 (mg/ml)
2	1	reservoir	HEPES	0.1 (M)
3	1	reservoir	magnesium acetate	0.2 (M)
4	1	reservoir	PEG8000	12-16 (%(w/v))